Directory: 02 Peak Broadening file name: 20_zero_signal

Symptom #

Zero signal with LC-MS/MS

Tool and Root Cause #

Tool: Visual Inspection
Root cause: Valve at ion source was closed

So basically, there is a valve that you close when you're doing this cleaning of this piece. You put this back in and you forgot to just switch that to it's open. So there's absolutely zero signal in this case, because there's no ion path.

Reference: MSACL. LC-MSMS Troubleshooting 101: Tips and Tricks for Getting Started : PART 1

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