Directory: 02 Peak Broadening file name: 01_peak_broadening
Note: There was no problems with mobile phase or needle wash or any method changes.
Symptom #
Troubleshooting Tool and Root Cause #
Troubleshooting Tool:
- Q: Check the Maintenance Log.
A: 615 injections. - Q: Check the system suitability tests results.
A: They like normal.
Root Cause: The guard column should be replaced at 500 injections, and there were 615 injections on it.
Reference:
MSACL. LC-MSMS Troubleshooting 101: Tips and Tricks for Getting Started : PART 1
MSACL: The Basics of LC-MSMS Troubleshooting: Tools, Strategy, Cases